Effect of dietary phytate on magnesium bioavailability and liver oxidant status in growing rats

Male albino rats (initial average weight 60 g) were fed semi-synthetic diets based on casein, corn starch and sunflower oil over 21 days. All diets were supplemented with 300 mg magnesium from MgSO4x7H2O and 0,7.5 and 15 g phytic acid (PA) from sodium phytate per kg. The addition of PA to the diets resulted in a dose-dependent decrease of apparent Mg absorption and Mg concentration in the plasma and femur. Impaired Mg bioavailability due to 15 g PA/kg diet was accompanied by an increase of hepatic thiobarbituric acid reactive substances and protein carbonyls as well as by a moderate decline in liver reduced glutathione (GSH) levels. The liver homogenates of rats receiving the diets with 7.5 and 15 g PA/kg, respectively, were much more susceptible to iron-induced lipid peroxidation than those of the controls. Hepatic antioxidative enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), glucose-6-phosphate dehydrogenase (G6PDH)], alpha-tocopherol concentration and phenyl-N-tert-butylnitrone (PBN) adducts using electron spin resonance spectroscopy remained unchanged by the different dietary treatments. Under the conditions of a marginal dietary Mg supply, phytate had pro-oxidative rather than antioxidative effects in the case of liver metabolism.