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Related Experiment Videos

Decrease in phosphoribulokinase activity by antisense RNA in transgenic tobacco. Relationship between photosynthesis,

Banks1, Driscoll, Parry

  • 1Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (F.M.B., S.P.D., M.A.J.P., D.W. L., M.J.P.).

Plant Physiology
|March 9, 1999
PubMed
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Altering phosphoribulokinase (PRK) activity in tobacco plants reveals that low nitrogen exacerbates the impact of reduced photosynthesis on biomass production. This highlights photosynthesis

Area of Science:

  • Plant Physiology
  • Biochemistry
  • Molecular Biology

Background:

  • Photosynthesis is a key process for plant growth and biomass accumulation.
  • Phosphoribulokinase (PRK) is a crucial enzyme in the Calvin cycle, regulating photosynthetic carbon fixation.
  • Understanding the direct impact of photosynthesis on biomass allocation is vital for crop improvement.

Purpose of the Study:

  • To investigate the direct effects of reduced photosynthesis on biomass allocation in transgenic tobacco.
  • To determine how nitrogen availability influences the impact of decreased PRK activity on plant growth.

Main Methods:

  • Generated transgenic tobacco plants with reduced phosphoribulokinase (PRK) activity using antisense technology.
  • Cultivated plants under varying nitrogen (N) levels (0.4 mM and 5 mM NH4NO3).

Related Experiment Videos

  • Assessed photosynthesis rates, PRK activity, biomass, starch accumulation, and leaf characteristics.

    • Main Results:

    • A 94% reduction in PRK activity had minimal impact on photosynthesis and growth at high N.
    • At low N, the same PRK reduction significantly decreased leaf (up to 50%) and whole-plant photosynthesis (up to 35%).
    • Plants with reduced PRK activity showed decreased biomass (up to 35%), structural dry matter, and starch content, particularly under low N.

    Conclusions:

    • Low nitrogen availability amplifies the negative effects of reduced PRK activity on photosynthesis and biomass production.
    • Photosynthesis limitations, even under conditions typically sink-limited, can significantly restrict biomass accumulation.
    • PRK activity is a critical determinant of plant biomass, especially under nutrient-limited conditions.