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The clinically isolated FIZ15 bacteriophage causes lysogenic conversion in Pseudomonas aeruginosa PAO1
S Vaca-Pacheco1, G L Paniagua-Contreras, O García-González
1Laboratorio de Genética, Escuela Nacional de Estudios Profesionales Iztacala, Universidad Nacional Autónoma de México, Apartado postal 314, Tlalnepantla, Edo. de Méx., 54090, México.
Abstract:
FIZ15 bacteriophage, from a human clinical isolate of Pseudomonas aeruginosa, causes lysogenic conversion in the P. aeruginosa strain PAO1. The prophage-conferred phenotypes are: (1) increased resistance to phagocytosis by mouse peritoneal macrophages; (2) increased resistance to killing by normal human serum, and (3) increased adhesion to human buccal epithelial cells. These phenotypes are related to the prophage-induced change at the level of its own bacterial receptor, which appears to be the O-antigen.
Insights
The FIZ15 bacteriophage confers Pseudomonas aeruginosa enhanced resistance to immune defenses and improved adhesion. These changes are linked to modifications in the bacterium's O-antigen receptor.
Area of Science:
- Microbiology
- Bacteriology
- Virology
Background:
- Pseudomonas aeruginosa is an opportunistic pathogen.
- Bacteriophages can alter bacterial properties through lysogenic conversion.
- Understanding these interactions is crucial for infection control.
Purpose of the Study:
- To investigate the effects of FIZ15 bacteriophage lysogenic conversion on Pseudomonas aeruginosa.
- To identify the mechanisms behind the conferred phenotypes.
Main Methods:
- Lysogenic conversion of P. aeruginosa strain PAO1 with FIZ15 bacteriophage.
- Assays for phagocytosis resistance using mouse peritoneal macrophages.
- Serum resistance assays using normal human serum.
- Adhesion assays with human buccal epithelial cells.
Main Results:
- FIZ15 lysogenic conversion resulted in increased resistance to phagocytosis.
- The converted strain showed enhanced resistance to normal human serum killing.
- Increased adhesion to human buccal epithelial cells was observed.
- These phenotypes are associated with alterations in the bacterial receptor, likely the O-antigen.
Conclusions:
- FIZ15 bacteriophage mediates significant phenotypic changes in Pseudomonas aeruginosa.
- The O-antigen appears to be the target of prophage-induced modification.
- Lysogenic conversion by FIZ15 enhances P. aeruginosa virulence and host interaction.