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Related Experiment Videos

Interstitial Ca2+ undergoes dynamic changes sufficient to stimulate nerve-dependent Ca2+-induced relaxation.

M M Mupanomunda1, N Ishioka, R D Bukoski

  • 1Section of Hypertension and Vascular Research, University of Texas Medical Branch, Galveston, Texas 77555-1065, USA.

The American Journal of Physiology
|March 10, 1999
PubMed
Summary

Interstitial calcium in the duodenum (Ca2+isf) fluctuates sufficiently to activate a sensory nerve pathway. This pathway influences regional resistance and organ blood flow by altering local calcium transport.

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Area of Science:

  • Physiology
  • Gastroenterology
  • Neuroscience

Background:

  • A perivascular sensory nerve-linked dilator system activated by interstitial calcium (Ca2+isf) has been previously described.
  • The functional significance of Ca2+isf levels in the duodenal submucosa remains to be fully elucidated.

Purpose of the Study:

  • To test the hypothesis that Ca2+isf in the rat duodenal submucosa varies within a range sufficient to activate the perivascular sensory nerve-linked dilator system.
  • To investigate the relationship between Ca2+isf and regional vascular resistance.

Main Methods:

  • In situ microdialysis was employed to estimate Ca2+isf in the rat duodenal submucosa under varying luminal calcium concentrations and feeding states.
  • Wire myography was utilized to assess the isometric tension responses of isolated mesenteric resistance arteries to extracellular calcium.

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Main Results:

  • Duodenal Ca2+isf levels demonstrated dynamic changes, ranging from 1.0 mmol/l in Ca2+-free buffer to 1.89 mmol/l with 10 mmol/l luminal Ca2+.
  • Ca2+isf was significantly higher in free-feeding rats (1.4 mmol/l) compared to fasted rats (1.1 mmol/l).
  • Extracellular calcium induced relaxation in pre-contracted mesenteric arteries with half-maximal effective doses of 1.54-1.67 mmol/l, indicating activation of a dilator pathway.

Conclusions:

  • Duodenal Ca2+isf fluctuates across a concentration range capable of activating the sensory nerve-linked dilator system.
  • This system serves as a crucial link between local calcium transport dynamics and alterations in regional vascular resistance and organ blood flow.