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[Basophil activation test using flow cytometry in Hymenoptera venom allergy]
S Stevens1, M Drouet, M G Lauret
1Laboratoire d'Immuno-Allergologie-C.H.U., Angers, France.
Allergie Et Immunologie
|March 10, 1999
Summary
The basophil activation test (TAB) effectively detects Hymenoptera venom allergies. Specific TAB values predict allergic reaction types, aiding in clinical management and patient care.
Area of Science:
- Immunology
- Allergy Diagnostics
Background:
- Hymenoptera venom immunotherapy requires accurate allergy diagnosis.
- Basophil activation testing (TAB) is a diagnostic tool for venom allergies.
Purpose of the Study:
- To establish a predictive threshold for TAB values in Hymenoptera venom allergies.
- To correlate TAB results with clinical reaction types (local vs. systemic).
Main Methods:
- Utilizing the basophil activation test (TAB) with three distinct dilutions.
- Comparing TAB values against patient clinical history and reaction types.
Main Results:
- TAB demonstrates clear evidence of Hymenoptera venom allergies.
- A threshold of 50% activation in the first dilution predicts reaction type.
- Below 50% activation, 70% of patients experienced local or systemic reactions.
- Above 50% activation, 70% of patients experienced systemic allergic reactions.
Conclusions:
- The basophil activation test (TAB) is a valuable tool for diagnosing Hymenoptera venom allergies.
- TAB thresholds can predict the type and severity of allergic reactions.
- This aids in tailoring treatment and managing patient outcomes effectively.