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Related Experiment Videos

Bicelle-based liquid crystals for NMR-measurement of dipolar couplings at acidic and basic pH values.

M Ottiger1, A Bax

  • 1Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520, USA.

Journal of Biomolecular NMR
|March 10, 1999
PubMed
Summary

New lipid mixtures form stable liquid crystalline phases, similar to bicelles, offering enhanced hydrolysis resistance. These findings are crucial for developing robust lipid-based systems for various applications.

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Area of Science:

  • Biochemistry
  • Materials Science
  • Structural Biology

Background:

  • Lyotropic liquid crystalline phases are essential for various biological and material applications.
  • Traditional phosphatidylcholine lipids can be susceptible to hydrolysis, limiting their stability.
  • Bicelles composed of dimyristoyl-phosphatidylcholine (DMPC) and dihexanoyl-phosphatidylcholine (DHPC) are widely used but have limitations.

Purpose of the Study:

  • To investigate novel lipid mixtures for forming stable lyotropic liquid crystalline phases.
  • To explore the use of alkyl analogs of phosphatidylcholine with ether linkages for improved hydrolytic stability.
  • To assess the structural integrity of ubiquitin under varying pH conditions using these new lipid systems.

Main Methods:

  • Preparation and characterization of lipid mixtures including ditetradecyl-phosphatidylcholine, didodecyl-phosphatidylcholine, and dihexyl-phosphatidylcholine in water.

Related Experiment Videos

  • Formation of lyotropic liquid crystalline phases under controlled conditions.
  • Nuclear Magnetic Resonance (NMR) spectroscopy, specifically 15N-1H dipolar couplings, to study protein structure.

    • Main Results:

    • Mixtures of alkyl analogs of phosphatidylcholine formed lyotropic liquid crystalline phases analogous to DMPC/DHPC bicelles.
    • The ether linkages in the alkyl analogs prevent acid- or base-catalyzed hydrolysis, enhancing compound stability.
    • Ubiquitin maintained a stable backbone conformation across a broad pH range (2.3-10.4) when studied using these lipid systems.

    Conclusions:

    • Novel lipid mixtures with ether linkages provide stable lyotropic liquid crystalline phases with improved hydrolytic resistance.
    • These findings offer a more stable alternative to traditional DMPC/DHPC bicelles.
    • The observed protein stability suggests the utility of these systems for structural studies under diverse conditions.