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Related Experiment Videos

Km (1) [Inv (1)] typing of saliva and semen.

M J Davie, A E Kipps

    Vox Sanguinis
    |November 1, 1976
    PubMed
    Summary

    This study details Km(1) typing using serum, saliva, and semen samples. Freezing and thawing saliva improves test accuracy by reducing viscosity, though some samples remain problematic.

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    Area of Science:

    • Forensic serology
    • Immunogenetics

    Background:

    • Accurate genetic typing from biological samples is crucial for identification.
    • Saliva and semen present unique challenges in serological analysis due to viscosity and composition.

    Purpose of the Study:

    • To report Km(1) typing results from matched serum, saliva, and semen samples.
    • To describe a method for improving saliva sample analysis for Km(1) typing.
    • To discuss challenges encountered during the typing process.

    Main Methods:

    • Km(1) typing was performed on 115 serum-saliva pairs and 31 serum-semen pairs.
    • Saliva samples were treated with freezing and thawing to decrease viscosity and prevent non-specific red cell aggregation.
    • Standard serological techniques were employed for typing.

    Main Results:

    • Successful Km(1) typing was achieved for a significant number of samples.
    • The freezing and thawing method effectively reduced saliva viscosity in most samples.
    • Certain saliva and semen samples did not respond optimally to the treatment, indicating potential limitations.

    Conclusions:

    • Km(1) typing is feasible using serum, saliva, and semen.
    • Pre-treatment of saliva by freezing and thawing is a valuable technique for enhancing typing reliability.
    • Further investigation is needed to address sample-specific issues affecting typing accuracy.

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