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Related Experiment Videos

CREB regulates MHC class II expression in a CIITA-dependent manner.

C S Moreno1, G W Beresford, P Louis-Plence

  • 1Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.

Immunity
|March 11, 1999
PubMed
Summary
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The study identifies CREB (cAMP response element-binding protein) as a key factor in MHC class II gene expression. CREB binds to the X2 box and works with CIITA to regulate these crucial immune genes.

Area of Science:

  • Immunology
  • Molecular Biology
  • Gene Regulation

Background:

  • MHC class II molecules are critical for immune responses.
  • The X2 box in MHC class II promoters is essential for gene expression.
  • The specific protein binding to the X2 box was previously unknown.

Purpose of the Study:

  • To identify the DNA-binding protein that interacts with the X2 box of MHC class II promoters.
  • To elucidate the role of this protein in the regulation of MHC class II gene expression.
  • To investigate the interaction between this protein and CIITA (Class II Transactivator).

Main Methods:

  • Purification and sequencing of the X2 box-binding protein (X2BP).
  • Transient transactivation assays to assess the functional role of X2BP.

Related Experiment Videos

  • Chromatin immunoprecipitation (ChIP) assay to confirm in vivo binding.
  • Experiments using ICER (Inducible cAMP Early Repressor) to study inhibition.

    • Main Results:

    • X2BP was identified as CREB (cAMP response element-binding protein).
    • CREB binds to the X2 box of MHC class II promoters.
    • CREB cooperates with CIITA to enhance MHC class II transcription in a dose-dependent manner.
    • CREB binding to the promoter in vivo was confirmed by ChIP.
    • ICER, an inhibitor of CREB, repressed MHC class II expression.

    Conclusions:

    • CREB is a crucial transcription factor that binds the X2 box in MHC class II promoters.
    • CREB collaborates with CIITA to regulate MHC class II gene expression.
    • This interaction is vital for directing the expression of MHC class II genes in vivo.