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[The expression of costimulating molecule B7.1 in lymphoma tissues]
Objective:
To study the expression of immuno-costimulating molecule B7.1 in lymphoma tissue and its part in immuno escape mechanism.
Methods:
Using the RT-PCR and in situ hybridization (ISH) techniques, the expression of B7.1 molecule in 12 fresh and 23 paraffin embedded tissues were studied.
Results:
(1) B7.1 mRNA transcription was detected in 10/12 lymphoma tissues by RT-PCR; (2) mRNA expression in 16/23 lymphoma tissues was detected by ISH with Dig-labelling B7.1 cDNA probe. Alternatively, a 450bp by transcript that was relevant to the normal B7.1 molecule was cloned and studied by RT-PCR and ISH respectively.
Conclusion:
More in depth studies should be performed on the relation between the transcript molecule and lymphoma and its effect on tumor immunity.