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[Microsatellite instability in nephroblastoma and its relationship to clinicopathological variables]
Objective:
To study the relationship between microsatellite instability (MSI) and clinicopathological variables in nephroblastoma.
Methods:
2 microsatellite repeat sequences AR (chromosome X), UT762(chromosome 21) were examined in normal and tumor pairs from 50 nephroblastoma patients by PCR and denatured polyacrylamide gel electrophoresis.
Results:
The MSI positive for AR and UT loci were 14/50 and 10/50 respectively in nephroblastoma. MSI positive for both AR and UT occurred in 2 cases. According to NTWS-III categorization, stage I & II belong to early stage and stage III & IV belong to late stage, the MSI for this series were 16/38 and 6/12 for early and late stages. According to the NWTI-II categorization, the MSI of this series for favorable and non-favorable histology were 71% and 33% respectively.
Conclusions:
Analysis of MSI may have some significance in discriminating the malignant status of nephroblastoma.