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Inhibitory effect of artemether on proteinase of Schistosoma japonicum
1Department of Clinical Pharmacology, Tongji Medical University, Wuhan, China.
Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica
|May 1, 1997
Aim:
To study the effect of artemether (Art) on the thio proteinase ("hemoglobinase", Hem) of Schistosoma japonicum.
Methods:
Hem was extracted from S japonicum adults. The inhibitory effect of Art on the activity of Hem to degrade human hemoglobin (Hgb) was examined with UV-photometer at 280 nm, SDS-PAGE and scanning at 600 nm on a chromoscanner.
Results:
Human Hgb was degraded at pH 4.0 by the Hem. The activities of Hem preincubated at 37 degrees C with Art 0.14, 1.4, and 14 mmol.L-1, were inhibited by 30.2%, 39.8%, and 45.0%, respectively.
Conclusion:
Art possesses an inhibitory effect to Hem of S japonicum.