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Effect of saponins of Panax notoginseng on synaptosomal 45Ca uptake
1Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College, Beijing, China.
Aim:
To explore the calcium uptake antagonism of saponins of Panax notoginseng (PNS).
Methods:
Synaptosomes were prepared from rat cerebral cortex by using differential Ficoll gradients. The effects of PNS on synaptosomal 45Ca uptake were measured in vitro or after acute treatment.
Results:
PNS 50-800 mg.L-1 produced a concentration-rated inhibition of Ca2+ uptake [IC50 = 111 (46-176) mg.L-1]. Both initial and maximal uptake were inhibited. Similar effect was obtained after acute PNS treatment with 200 mg.kg-1 i.p. The blocking effect of PNS was reversed by calcium in media.
Conclusion:
PNS is a calcium channel blocker in neurons.