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Monoclonal antibody 3F3 against conformational epitope of Torpedo acetylcholinesterase
1Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing, China.
Aim:
To study the type of epitope of native Torpedo acetylcholinesterase (AChE) directed by its monoclonal antibody (McAb) 3F3.
Methods:
Enzyme-linked immunosorbent assay (ELISA) was used for the assay of the reaction between antigen and antibody.
Results:
McAb 3F3 immunoreacted well with the native AChE, but not with the reduced- and alkylated-AChE (RA-AChE) at all. Soman did not interfere the binding of 3F3 with AChE molecule. The synthesized 24-peptide containing the active serine residue of the AChE active center did not react with McAb 3F3.
Conclusion:
3F3 is a monoclonal antibody against the conformational epitope of Torpedo AChE active center, but dose not occupy the active serine residue of the enzyme.