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[Neoplasm-inhibiting effect and sensitivity-promoting effect of indomethacin in vitro]
1Department of Alimentary Disease, Xiangya Hospital, Hunan Medicine University, Changsha.
Hunan Yi Ke Da Xue Xue Bao = Hunan Yike Daxue Xuebao = Bulletin of Hunan Medical University
|January 1, 1997
Abstract
Objective:
To explore an anti-tumor effect of indomethacin on human colon adenocarcinoma cell line HCT116, and its mechanism.
Methods:
MTT, agarose gel electrophoresis, TdT and flow cytometry were used to study cell proliferation and apoptosis.
Results:
Indomethacin significantly inhibited the proliferation of HCT116 cells and induced apoptosis with a "dose-time" dependent pattern. A synergic effect of inhibiting cancer cells proliferation was observed when combined with 5-Fu.
Conclusion:
Inhibition of proliferation and induction of apoptosis contribute to the mechanisms for anti-tumor activity of indomethacin.