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[Rapid bioluminescence method for pathogen detection of nephropyelitis patient]
1Department of Clinical Laboratory, Xiangya Hospital, Hunan Medical University, Changsha.
Hunan Yi Ke Da Xue Xue Bao = Hunan Yike Daxue Xuebao = Bulletin of Hunan Medical University
|January 1, 1997
Summary
Bioluminescence detection of adenosine triphosphate (ATP) in urine accurately identifies bacterial infections. This sensitive method aids in the rapid diagnosis of L-form bacteriuria, distinguishing infected patients from non-infected ones.
Area of Science:
- Biochemistry
- Microbiology
- Medical Diagnostics
Context:
- Urinary tract infections (UTIs) are common, necessitating accurate and rapid diagnostic methods.
- Traditional urine culture methods can be time-consuming, delaying appropriate treatment.
- The presence of adenosine triphosphate (ATP) indicates metabolically active cells, including bacteria.
Purpose:
- To evaluate the efficacy of bioluminescence assay for detecting ATP in urine as a diagnostic tool for bacteriuria.
- To compare the sensitivity and specificity of the bioluminescence method with conventional diagnostic techniques like Gram Stain and bacterial cultures.
- To determine diagnostic thresholds for ATP levels indicative of infection.
Summary:
- A bioluminescence method was employed to measure ATP content in urine samples from 222 patients.
- Significantly higher mean ATP levels (> 130.9 +/- 11.38) were observed in infected patients compared to non-infected patients (< 32.9 +/- 2.26).
- Results correlated well with Gram Stain and both common and L-form bacteriuria cultures, demonstrating high sensitivity.
Impact:
- The bioluminescence method offers a rapid and highly sensitive approach for detecting bacteriuria, including L-form variants.
- This technique facilitates quicker diagnosis of urinary tract infections, potentially improving patient outcomes.
- Early and accurate diagnosis through bioluminescence can guide timely medical intervention.