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Published on: June 8, 2011
[Determination of inorganic phosphate in serum by double reagents with Hitachi 7170 A analyzer]
1Department of Clinical Laboratory, Second Affiliated Hospital, Hunan Medical University, Changsha.
Abstract:
We propose a method for determination of inorganic phosphate in serum using double reagents with Hitachi 7170 A analyzer. The standard curve for phosphate was linear from 1.0-5.0 mmol.L-1. Both coefficient of accuracy and precision were above 95%. The recovery rate ranged from 98.2%-99.5%. Jaundice Sample and lipohemia serum were found to be interference--free. This method can eliminate paraprotein interference, it was an ideal method for determination of inorganic phosphate in serum.

