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Related Experiment Video

Updated: Jul 16, 2026

RNAi Interference by dsRNA Injection into Drosophila Embryos

RNAi Interference by dsRNA Injection into Drosophila Embryos

Published on: April 11, 2011

Truncated RanGAP encoded by the Segregation Distorter locus of Drosophila.

C Merrill¹, L Bayraktaroglu, A Kusano

¹Laboratory of Genetics, 445 Henry Mall, University of Wisconsin, Madison, WI 53706, USA.

Science (New York, N.Y.)

|March 12, 1999

Summary

Segregation Distorter (SD) in Drosophila melanogaster causes excess transmission of the SD chromosome by inducing spermatid dysfunction. Researchers identified a mutant RanGAP protein as the functional product responsible for this meiotic drive.

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Area of Science:

Genetics
Molecular Biology
Evolutionary Biology

Background:

Segregation Distorter (SD) is a naturally occurring meiotic drive system in Drosophila melanogaster.
SD causes the SD chromosome to be transmitted in excess over its homolog (SD+) in males.
This excess transmission results from induced dysfunction of SD+-bearing spermatids.

Purpose of the Study:

To identify the key gene responsible for the meiotic drive phenotype.
To determine the functional product of the Sd locus.

Main Methods:

Germline transformation was used to introduce a genomic fragment from the Sd region into Drosophila.
The distorting activity of the transformed flies was assessed.

Main Results:

A genomic fragment from the Sd region conferred full distorting activity.
The only functional product encoded by this fragment was a truncated version of the RanGAP nuclear transport protein.

Conclusions:

The mutant RanGAP protein is the functional product of the Sd locus.
This finding elucidates the molecular mechanism underlying Segregation Distorter meiotic drive.