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Endogenous endophthalmitis by Fusarium solani: an animal experimental model
1Servei Patologia, Hospital Universitari de Tarragona Joan XXIII, Spain.
Abstract:
An experimental model of endophthalmitis by Fusarium solani in immunocompetent mice that could be useful for evaluating the efficacy of different treatments and the pathogenicity of the fungus in ocular structures was established. Five clinical isolates of F. solani were injected into the lateral tail vein of groups of 20 mice, in order to produce systemic infection with ocular infection. Inocula of 5 x 10(6) conidia per mouse were used. The eyes of the animals that died were enucleated for histopathological study to determine the degree of ocular infection. We found fungal infections in 34% of the mice studied. Panophthalmitis was detected in 16 animals, four with bilateral infections. Fungal endophthalmitis can become a severe complication of systemic mycoses by F. solani.
Insights
Researchers developed a mouse model for Fusarium solani endophthalmitis, a serious eye infection. This model aids in testing treatments and understanding the fungus
Area of Science:
- Ophthalmology
- Mycology
- Infectious Diseases
Background:
- Fusarium solani is a fungal pathogen capable of causing severe ocular infections.
- Endophthalmitis, an inflammation inside the eye, can lead to vision loss.
- Systemic fungal infections can result in ocular complications.
Purpose of the Study:
- To establish an experimental model of Fusarium solani endophthalmitis in immunocompetent mice.
- To evaluate the pathogenicity of F. solani in ocular structures.
- To provide a model for testing the efficacy of potential treatments.
Main Methods:
- Five clinical isolates of Fusarium solani were used.
- Inocula of 5 x 10^6 conidia per mouse were injected intravenously into immunocompetent mice.
- Ocular infection was assessed through histopathological study of enucleated eyes from deceased animals.
Main Results:
- A systemic infection model with ocular involvement was successfully established.
- Fungal infections were detected in 34% of the studied mice.
- Panophthalmitis (inflammation of all layers of the eye) was observed in 16 mice, with four exhibiting bilateral infections.
Conclusions:
- The experimental mouse model effectively replicates Fusarium solani endophthalmitis.
- Fusarium solani can cause severe ocular infections, including panophthalmitis, as a complication of systemic mycosis.
- This model is valuable for future research into F. solani ocular infections and treatment strategies.