Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sec24 proteins and sorting at the endoplasmic reticulum.

A Pagano1, F Letourneur, D Garcia-Estefania

  • 1Department of Morphology, University Medical Center, Geneva University, Geneva CH-1211, Switzerland.

The Journal of Biological Chemistry
|March 13, 1999
PubMed
Summary

Researchers identified a family of Sec24 proteins involved in secretory vesicle formation. These proteins play a role in selecting and concentrating cargo for secretion from the endoplasmic reticulum.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel approaches for understanding and improving the effectiveness of seed biopriming.

Scientific reports·2026
Same author

Evaluating a Tobacco-free Policy Intervention in Residential Substance Use Disorder Programs.

Journal of drug issues·2026
Same author

Study of neutron efficiency and cross-talk effects through Monte Carlo simulations for a new neutron detector NArCoS.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2026
Same author

Sex-difference of multifactorial intervention on cardiovascular and mortality risk in DKD: post-hoc analysis of a randomised clinical trial.

Cardiovascular diabetology·2024
Same author

RadioLab project: knowledge of radon gas in Italy.

Scientific reports·2024
Same author

Blood lactate in mild and moderate ARDS secondary to SARS COV 2.

The American journal of emergency medicine·2023

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Trafficking

Background:

  • COPII proteins are essential for forming secretory vesicles at the endoplasmic reticulum.
  • Yeast possesses two orthologues of the Sec24 protein, a key COPII component.

Purpose of the Study:

  • To investigate the function of Sec24 orthologues in protein secretion.
  • To identify and characterize the human Sec24 protein family and their role in vesicle formation.

Main Methods:

  • Gene knock-out in yeast to study secretion defects.
  • Database searches to identify homologous proteins across species.
  • Cloning of human cDNAs encoding Sec24 homologues.
  • Co-immunoprecipitation and size exclusion chromatography to analyze protein complexes.

Related Experiment Videos

  • Immunofluorescence microscopy to determine protein localization.

    • Main Results:

    • Absence of a specific Sec24 orthologue in yeast caused selective protein secretion defects.
    • A family of Sec24-related proteins exists in humans, worms, flies, and plants.
    • Two new human cDNAs encoding Sec24p homologues were identified.
    • Human Sec24 orthologues form complexes with hSec23Ap and co-localize with hSec23p and hSec13p.

    Conclusions:

    • The Sec23-24p complex is involved in cargo selection and concentration during vesicle formation.
    • Sec24 proteins are crucial for efficient protein secretion via secretory vesicles.