Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Myocardial cell hypertrophy after myocardial infarction with reperfusion in dogs.

Circulation·1992
Same author

Disappearance of Q waves following thrombolysis for acute myocardial infarction.

The Canadian journal of cardiology·1992
Same author

Myxomatosis: population dynamics of rabbits (Oryctolagus cuniculus Linnaeus, 1758) and ecological effects in the United Kingdom.

Revue scientifique et technique (International Office of Epizootics)·1992
Same author

Identification of complex formation between two intracellular tyrosine kinase substrates: human c-Rel and the p105 precursor of p50 NF-kappa B.

Oncogene·1992
Same author

Improved processes for the production and isolation of dynemicin A and large-scale fermentation in a 10,000-liter fermentor.

Journal of industrial microbiology·1992
Same author

The effect of postural drainage positioning on ventilation homogeneity in healthy subjects.

Physical therapy·1992
Same journal

Mapping protein-DNA interactions across biological scales: principles, scalability and applications.

Methods (San Diego, Calif.)·2026
Same journal

Establishing a standard workflow for fluorescence-based nanoparticle tracking analysis for the reliable quantification of extracellular vesicles.

Methods (San Diego, Calif.)·2026
Same journal

Quantitative single-cell analysis of PML-RARα oncogene-induced DNA damage along cell cycle progression.

Methods (San Diego, Calif.)·2026
Same journal

Cilia SubQ: a modular suite of semi- and fully automated pipelines for analysis of primary cilia and ciliary subdomains.

Methods (San Diego, Calif.)·2026
Same journal

Projective invariant of surface ratio: application to pupil measurement through simulations and proof-of-concept recordings.

Methods (San Diego, Calif.)·2026
Same journal

A quantitative radiographic framework for longitudinal monitoring of additively manufactured biodegradable scaffolds with graded tantalum reinforcement.

Methods (San Diego, Calif.)·2026
See all related articles

Related Experiment Video

Updated: Jun 29, 2026

A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases
06:10

A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases

Published on: December 23, 2013

Assays for analyzing exonucleases in vitro.

J Ross1

  • 1McArdle Laboratory for Cancer Research, 1400 University Avenue, Madison, Wisconsin 53706, USA.

Methods (San Diego, Calif.)
|March 17, 1999
PubMed
Summary
This summary is machine-generated.

This study details exoribonucleases, enzymes crucial for RNA degradation. Methods for assaying both 5' to 3' and 3' to 5' exonucleases are provided, including a mammalian 3' to 5' exonuclease extract.

More Related Videos

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
05:33

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

Related Experiment Videos

Last Updated: Jun 29, 2026

A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases
06:10

A Fluorescence-based Exonuclease Assay to Characterize DmWRNexo, Orthologue of Human Progeroid WRN Exonuclease, and Its Application to Other Nucleases

Published on: December 23, 2013

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
05:33

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

Area of Science:

  • Molecular Biology
  • Enzymology

Background:

  • Ribonucleases are vital for cellular processes like growth, differentiation, and stress response.
  • Exoribonucleases specifically degrade RNA from the 5' or 3' end.

Purpose of the Study:

  • To describe the preparation of a mammalian 3'-to-5' exonuclease crude extract.
  • To provide assay conditions for both 5'-to-3' and 3'-to-5' exoribonucleases.

Main Methods:

  • Preparation of a crude extract from mammalian cells.
  • Development of assay conditions for enzymatic activity of exoribonucleases.
  • Characterization of yeast and vertebrate exoribonucleases.

Main Results:

  • Successful preparation of a mammalian 3'-to-5' exonuclease crude extract.
  • Established assay conditions applicable to various exoribonucleases.
  • Identified a yeast exoribonuclease involved in mRNA decay and vertebrate exoribonucleases with potential roles in mRNA stability.

Conclusions:

  • The study provides practical methods for studying exoribonucleases.
  • The findings contribute to understanding RNA degradation pathways and mRNA stability mechanisms.