Dependence of yeast mitochondrial unselective channel activity on the respiratory chain

The dependence of yeast mitochondrial unselective channel activity on the respiratory chain was investigated. Modulation of the respiratory chain with different substrates and inhibitors showed that channel activity was dependent on the electron flow rate through the chain and that external NADH only could provide a sufficient rate to activate the channel. These results support the hypothesis that the yeast mitochondrial unselective channel may be involved in the oxidation of cytosolic NADH without coupling to ATP synthesis.