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Electrochemical immunosensor for the detection of atrazine
M A López1, F Ortega, E Domínguez
1Departamento de Química Analítica, Facultad de Farmacia, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
Journal of Molecular Recognition : JMR
|March 17, 1999
Abstract:
An amperometric immunosensor for the detection of the herbicide atrazine has been developed. A redox polymer PVPOs(bpy)2Cl was co-immobilized with the specific antibody on the surface of the electrode by crosslinking with PEGDGE to form an electron-conducting hydrogel. In a competitive assay the occurrence of the antibody-antigen reaction on the surface of the sensing film was detected through the 'electrical wiring' of the redox centres of antigen-labelled horseradish peroxidase and the electrode surface in the presence of H2O2 at 0.1 V (vsAg/AgCl).