The GABA(C) receptor is present in cone-horizontal cell axon terminals isolated from catfish retina

Whole cell voltage-clamp recordings were performed on isolated terminals and somata from catfish retina to compare the distribution of excitatory and inhibitory receptors in both structures. Saturating concentrations of glutamate or kainate produced small currents in axon terminals, averaging less than 8% of the current evoked in the soma. In contrast, application of high concentrations of gamma-aminobutyric acid (GABA) produced approximately similar current amplitudes in both structures. Based on estimates of membrane surface area, GABA-induced current densities were around 0.05 pA/microm2 for both structures. The GABA-activated current in the axon terminal was not blocked by bicuculline or SR95531, but was completely inhibited by picrotoxin. Baclofen did not mimic the GABA effect, but trans-4-aminocrotonic acid (TACA, 300 microM) and muscimol (1 mM) elicited currents of 100 and 40 pA, respectively. These results suggest that the axon terminals of cone-horizontal cells possess GABA(C) receptors at a high density, do not possess GABA(A) or GABA(B) receptors, and have few glutamate receptors. The GABA(C) receptors could function as postsynaptic receptors in the inner plexiform layer or as autoreceptors.