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[Identification of conditionally pathogenic enterobacteria in laboratory practice in the diagnosis of acute
Abstract:
Identification of 361 cultures isolated from patients suffering from various acute intestinal diseasesand from persons who had sustained them, as well as from contacts and persons examined prophylactically with the use of various biochemical tests showed that ty their taxonomic properties the cultures were referred to conditionally-pathogenic representatives of Enterobacteriaceae of the corresponding genera:Citrobacter, Hafnia, Klebsiella, Proteus, Providencia. Serological typing of the strains of bacteria of the Citrobacter pointed to the most frequent circulation of the strains of the serological groups 04, 01, 03, 013, 05, 022, 08; among these groups 04, encountered among all the categories under study, prevailed. At the current stage of identification of conditionally pathogenic enterobacteria in practical laboratories it is of expedience to use in the diagnosis of intestinal diseases the serological typing along with a complex from several additional biochemical tests.
Insights
This study identified 361 conditionally pathogenic Enterobacteriaceae from intestinal disease patients and contacts. Serological typing revealed Citrobacter group 04 as the most prevalent, supporting its use alongside biochemical tests for diagnosis.
Area of Science:
- Microbiology
- Clinical Diagnostics
- Bacteriology
Context:
- Acute intestinal diseases pose a significant public health challenge.
- Accurate identification of causative agents is crucial for effective treatment and control.
- Enterobacteriaceae are common causes of gastrointestinal infections.
Purpose:
- To identify and classify conditionally pathogenic Enterobacteriaceae from patients with acute intestinal diseases and their contacts.
- To determine the prevalence of specific serological groups within the Citrobacter genus.
- To evaluate the utility of serological typing combined with biochemical tests for diagnosing intestinal diseases.
Summary:
- Biochemical tests identified 361 cultures as conditionally pathogenic Enterobacteriaceae (Citrobacter, Hafnia, Klebsiella, Proteus, Providencia) from various patient and contact groups.
- Serological typing of Citrobacter strains indicated the frequent circulation of serogroups 04, 01, 03, 013, 05, 022, and 08, with 04 being the most prevalent across all groups.
- The study advocates for the combined use of serological typing and multiple biochemical tests in practical laboratory settings for diagnosing intestinal diseases.
Impact:
- Provides insights into the epidemiological distribution of key Enterobacteriaceae in intestinal infections.
- Highlights the diagnostic value of integrating serological methods with biochemical analysis for improved accuracy.
- Contributes to better understanding and management of bacterial gastroenteritis.