[High throughput gene analysis with DNA array--from sequencing to expression monitoring]

Proceedings of DNA array technology opened the way for the high throughput gene analysis, such as sequence analysis by hybridization (SBH) or gene expression monitoring. SBH, based on the ability to determine a DNA sequence by hybridizing oligonucleotide probes to the target DNA, appears to be a method with great potential for megabase sequencing. One of the most serious problems is that SBH requires extremely highly specific hybridization conditions which can discriminate one-base mismatches against a perfect match. We proposed new probe designs that contain several universal bases at both ends. By using these stacking probes, we could obtain not only strong hybridization signals, but also high discrimination values.