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ISWI is an ATP-dependent nucleosome remodeling factor
D F Corona1, G Längst, C R Clapier
1European Molecular Biology Laboratory, Heidelberg, Germany.
Molecular Cell
|March 17, 1999
Summary
The isolated ISWI ATPase protein can remodel nucleosomes and assemble chromatin independently. This suggests ISWI
Area of Science:
- Molecular Biology
- Chromatin Biology
- Biochemistry
Background:
- The ATPase ISWI is a core component of nucleosome remodeling complexes.
- These complexes are crucial for altering DNA accessibility within chromatin.
Purpose of the Study:
- To investigate the intrinsic nucleosome remodeling capabilities of the isolated ISWI protein.
- To determine if ISWI requires other subunits for its remodeling activity.
Main Methods:
- Biochemical assays to assess nucleosome remodeling.
- Assays for nucleosome rearrangement and chromatin assembly.
- Enzyme kinetics to study ATPase activity stimulation.
Main Results:
- The isolated ISWI protein demonstrated nucleosome remodeling, rearrangement, and chromatin assembly functions.
- ISWI's ATPase activity was significantly stimulated by nucleosomes, but not free DNA or histones.
- These findings indicate ISWI recognizes specific nucleosome structural features.
Conclusions:
- Nucleosome remodeling by ISWI does not necessitate interaction with other subunits.
- Associated proteins likely regulate ISWI activity or its cellular context.
- ISWI possesses intrinsic catalytic activity for chromatin manipulation.