Determination of ifosfamide by HPLC using on-line sample preparation

Area of Science:

• Analytical Chemistry
• Pharmacology
• Oncology

Background:

• Ifosfamide (IF) is a crucial chemotherapeutic agent, particularly for osteosarcoma.
• Accurate determination of IF levels is essential for effective treatment and patient monitoring.
• Existing methods for IF quantification can be complex and time-consuming.

Purpose of the Study:

• To develop and validate a novel, automated, and user-friendly High-Performance Liquid Chromatography (HPLC) method for ifosfamide determination.
• To assess the method's applicability in a clinical setting, specifically in osteosarcoma patients receiving IF therapy.

Main Methods:

• Development of an HPLC method incorporating on-line sample processing and automated solid-phase extraction.
• Integration of an automatic preparator with the chromatographic system for streamlined analysis.
• Validation of the method's linearity and sensitivity, establishing a calibration range and minimum detectable concentration.

Main Results:

• The developed HPLC method demonstrated linearity within the 6-200 microg/ml concentration range.
• A minimum detectable concentration of 6 microg/ml was achieved, indicating high sensitivity.
• The method proved to be highly accurate and easily reproducible, suitable for routine clinical use.

Conclusions:

• The novel automated HPLC method offers a reliable and efficient approach for ifosfamide quantification.
• This method facilitates precise monitoring of ifosfamide levels during osteosarcoma treatment with slow infusion protocols.
• The ease of application and high accuracy make this method valuable for clinical laboratories and oncologists.