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Updated: Aug 10, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Effect of salt concentration and pH on the hydrophobicity parameters of surfactants studied by TLC and spectral
T Cserháti1, E Forgács, G Oros
1Central Research Institute for Chemistry, Hungarian Academy of Sciences, Budapest.
Abstract:
The lipophilicity and specific hydrophobic area of 56 surfactants having different hydrophobic moiety and different length of ethylene oxide chain were determined by reversed-phase thin-layer chromatography and the strength and selectivity of the effect of sodium chloride and pH on the hydrophobicity parameters was elucidated using spectral mapping technique followed by two-dimensional nonlinear mapping. In each instance significant linear correlations were found between the lipophilicity and specific hydrophobic surface area of surfactants suggesting that from a chromatographic point of view they behave as a homologous series of solutes. It was established that the strength of the effect of both salt concentration and pH is relatively low and the selectivity of their influence on the hydrophobicity parameters is markedly different.
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