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Related Experiment Videos

RPD3 (REC3) mutations affect mitotic recombination in Saccharomyces cerevisiae.

E G Dora1, N Rudin, J R Martell

  • 1Department of Biology, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132, USA.

Current Genetics
|March 18, 1999
PubMed
Summary
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The RPD3 gene, encoding a histone deacetylase, influences both gene expression and DNA repair in yeast. Mutations in RPD3 affect sporulation and reduce spontaneous mitotic recombination, impacting chromatin architecture.

Area of Science:

  • Yeast genetics
  • Molecular biology
  • Epigenetics

Background:

  • The Saccharomyces cerevisiae rec3-1ts mutation affects mitotic recombination and sporulation.
  • This mutation is located in the RPD3 gene, which encodes a histone deacetylase.

Purpose of the Study:

  • To investigate the role of RPD3 in regulating gene expression and mitotic recombination.
  • To characterize the function of the rec3-1ts allele and RPD3 deletions.

Main Methods:

  • Complementation analysis of the rec3-1ts mutation with a genomic fragment.
  • Assaying sporulation and acid-phosphatase activity in various yeast strains.
  • Measuring spontaneous mitotic recombination events in different genetic backgrounds.

Main Results:

Related Experiment Videos

  • The complementing fragment was identified as RPD3.
  • The rec3-1ts allele confers reduced sporulation and increased acid-phosphatase activity.
  • RPD3 deletion mutants showed reduced spontaneous mitotic recombination.
  • RPD3 function is not cell-cycle regulated.

Conclusions:

  • RPD3 plays a critical role in regulating gene expression and mitotic recombination in yeast.
  • Changes in chromatin architecture mediated by RPD3 impact these cellular processes.
  • The rec3-1ts allele represents a hypomorphic mutation of RPD3.