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Starvation in yeast increases non-adaptive mutation.

A Marini1, N Matmati, G Morpurgo

  • 1Istituto di Anatomia comparata, University of Perugia, Via Pascoli, I-06100 Perugia, Italy.

Current Genetics
|March 18, 1999
PubMed
Summary
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Histidine starvation significantly increases mutation frequencies in yeast cells. This starvation effect enhances spontaneous and mutagen-induced (6-N-hydroxylaminopurine) reversion rates in stationary cells.

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Investigating mutation frequency changes in yeast Saccharomyces cerevisiae.
  • Understanding the impact of nutrient starvation on genetic stability.

Purpose of the Study:

  • To determine if histidine starvation affects reversion frequencies in yeast mutants.
  • To examine the role of starvation in non-adaptive mutagenesis.
  • To assess the interaction between starvation and a base analogue mutagen.

Main Methods:

  • Utilizing histidine-requiring and tryptophan-requiring mutants of Saccharomyces cerevisiae.
  • Inducing histidine starvation in stationary phase cells.
  • Assessing reversion frequencies under starvation conditions.
  • Treating cells with 6-N-hydroxylaminopurine (HAP) before or during starvation.

Related Experiment Videos

Main Results:

  • Histidine starvation increased reversion frequency about ten-fold in histidine-requiring mutants.
  • Similar increases in reversion frequency were observed in tryptophan-requiring mutants under histidine starvation.
  • The base analogue 6-N-hydroxylaminopurine (HAP) strongly increased reversion, with this effect further enhanced in stationary starving cells.
  • HAP added to already starving stationary cells showed no additional effect.

Conclusions:

  • Nutrient starvation, specifically histidine starvation, enhances mutation frequencies in yeast in a non-adaptive manner.
  • Starvation conditions potentiate the mutagenic effects of base analogues like HAP.
  • The timing of mutagen exposure relative to starvation is critical for its effect on mutation rates.