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Enhanced hatching rate of bovine IVM/IVF/IVC blastocysts using a 1.48-micron diode laser beam
1Maria Infertility Medical Institute, Sinseol-Dong Dongdaemun-Gu, Seoul, Korea.
Purpose:
Our purpose was to test whether zona pellucida (ZP) drilling using a 1.48-micron diode laser beam on bovine IVM/IVF/IVC blastocysts is effective for embryo hatching.
Methods:
Blastocysts produced in vitro at day 7 after IVF were divided into control and laser-drilled groups, respectively.
Results:
When the rates of in vitro development of bovine embryos were examined, the average cleavage rate (> or = two-cell) was 82.3% and the blastocyst rate at day 7 after IVF was 32.5%. Using these blastocysts, when the laser drilling effect was investigated at 48 hr after treatment, the total hatching rate in the laser-drilled group (98.0%) was significantly higher than that in the control group (60.0%) (P < 0.001). Especially, the hatched rate of the laser-drilled group (68.0%) was significantly enhanced compared with that of the control group (30.0%) (P < 0.001).
Conclusions:
These results demonstrated that laser ZP drilling on bovine IVM/IVF/IVC blastocysts can significantly increase the hatching rate.