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Related Experiment Videos

Improved high-performance liquid chromatographic assay for nimesulide.

D J Jaworowicz1, M T Filipowski, K M Boje

  • 1Department of Pharmaceutics, School of Pharmacy, University at Buffalo, NY 14260, USA.

Journal of Chromatography. B, Biomedical Sciences and Applications
|March 18, 1999
PubMed
Summary

A new, validated High-Performance Liquid Chromatography (HPLC) assay for nimesulide offers improved efficiency. This method uses less plasma and a similar compound, NS-398, for accurate analysis in pharmacokinetic studies.

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Area of Science:

  • Analytical Chemistry
  • Pharmacology

Background:

  • Nimesulide is a widely used non-steroidal anti-inflammatory drug (NSAID).
  • Accurate quantification of nimesulide in biological matrices is crucial for pharmacokinetic studies.
  • Existing analytical methods may have limitations in terms of sample volume or efficiency.

Purpose of the Study:

  • To develop and validate an improved High-Performance Liquid Chromatography (HPLC) assay for the quantification of nimesulide in plasma.
  • To establish a method that requires minimal plasma volume and utilizes a readily available internal standard.

Main Methods:

  • Development of a liquid-liquid extraction protocol using toluene and aqueous base.
  • Reversed-phase HPLC analysis on a Shandon Hypersil BDS C18 column.
  • Utilized NS-398, a structurally similar analogue, as the internal standard.

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Main Results:

  • The developed HPLC assay requires only 0.2 ml of plasma.
  • The entire extraction and analysis procedure can be completed within 4 hours.
  • Achieved a chromatographic run time of 12 minutes with distinct retention times for nimesulide and NS-398.

Conclusions:

  • The validated HPLC assay is efficient and reliable for nimesulide quantification.
  • The method's reduced sample volume requirement makes it suitable for various pharmacokinetic studies.
  • The assay was successfully applied in a pilot pharmacokinetic study, demonstrating its practical utility.