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Amplification of the c-yes oncogene in canine mammary tumors
A Rungsipipat1, S Tateyama, R Yamaguchi
1Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University, Japan.
Abstract:
Genomic DNAs of 14 mammary tumors were analyzed by Southern blot hybridization using a human c-yes-1 oncogene probe. The amplification was successful in half of the cases (7 adenocarcinomas). The degree of amplification was approximately 4-fold, and a high proportion was seen in malignant tumors. In addition, DNA polymorphism was detected in two adenocarcinomas.
Insights
Genomic analysis revealed amplification of the c-yes-1 oncogene in half of mammary tumors studied, particularly in malignant adenocarcinomas. This genetic alteration, detected via Southern blot, suggests a role for c-yes-1 in mammary cancer progression.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The c-yes-1 oncogene is implicated in cellular growth and differentiation.
- Aberrant oncogene expression is a hallmark of many cancers, including mammary tumors.
Purpose of the Study:
- To investigate the presence and extent of c-yes-1 oncogene amplification in human mammary tumors.
- To correlate c-yes-1 amplification with tumor malignancy and DNA polymorphism.
Main Methods:
- Southern blot hybridization was employed to analyze genomic DNA from 14 human mammary tumors.
- A human c-yes-1 oncogene probe was used to detect gene amplification and polymorphism.
Main Results:
- c-yes-1 gene amplification was identified in 7 out of 14 (50%) mammary tumors, specifically in adenocarcinomas.
- The amplification level was approximately 4-fold, with a higher prevalence observed in malignant tumors.
- DNA polymorphism of the c-yes-1 gene was detected in two adenocarcinoma cases.
Conclusions:
- The findings suggest that c-yes-1 oncogene amplification is a recurring event in a subset of human mammary tumors.
- Amplification of c-yes-1 may be associated with the malignant phenotype of mammary adenocarcinomas.
- Further research is warranted to elucidate the functional significance of c-yes-1 amplification in mammary tumorigenesis.