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Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and
C Kinsland1, S V Taylor, N L Kelleher
1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.
Protein Science : a Publication of the Protein Society
|March 19, 1999
Abstract:
A facile and rapid method for the production of protein C-terminal thiocarboxylates on DNA-encoded polypeptides is described. This method, which relies on the mechanism of the cleavage reaction of intein-containing fusion proteins, can produce multi-milligram quantities of protein C-terminal thiocarboxylate quickly and inexpensively. The utility of this method for protein semisynthesis and implications for studies on the biosynthesis of thiamin are discussed.