Related Experiment Video

Updated: Oct 24, 2026

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact

Published on: July 17, 2016

Protective effects of the TNF-ceramide pathway against glutamate neurotoxicity on cultured mesencephalic neurons

K Shinpo¹, S Kikuchi, F Moriwaka

¹Department of Neurology, Hokkaido University Hospital, Kita 14, Nishi 5, Kitaku, Sapporo, Japan. kshinpo@med.hokudai.ac.jp

|March 20, 1999

Abstract:

Pretreatments with TNF-alpha and lower concentrations of C2-ceramide protected cultured mesencephalic neurons from excitotoxicity in a dose-dependent manner. These protective effects are reduced by cotreatment with N,N-dimethylsphingosine (DMS), an inhibitor of sphingosine kinase. Since the pretreatment with sphingosine-1-phosphate (SPP) showed a neuroprotective effect, our data suggest that protective effects of TNF and C2-ceramide could be attributable to their further metabolism to SPP.

More Related Videos

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Published on: August 15, 2012

Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons

Quantifying Spontaneous Ca²⁺ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons

Published on: September 9, 2020

Related Concept Videos

Articles linked to this work by shared authors, journal, and citation graph.

Prostaglandin E1 protects cultured spinal neurons against the effects of nitric oxide toxicity.

Neuropharmacology·2002

[The advance in the research and therapeutic trials of amyotrophic lateral sclerosis].

Rinsho shinkeigaku = Clinical neurology·2001

[Diagnostic criteria for Parkinson's disease].

Nihon rinsho. Japanese journal of clinical medicine·2000

Effect of 1,25-dihydroxyvitamin D(3) on cultured mesencephalic dopaminergic neurons to the combined toxicity caused by L-buthionine sulfoximine and 1-methyl-4-phenylpyridine.

Journal of neuroscience research·2000

Selective vulnerability of spinal motor neurons to reactive dicarbonyl compounds, intermediate products of glycation, in vitro: implication of inefficient glutathione system in spinal motor neurons.

Brain research·2000

Detection of an Amadori product, 1-hexitol-lysine, in the anterior horn of the amyotrophic lateral sclerosis and spinobulbar muscular atrophy spinal cord: evidence for early involvement of glycation in motoneuron diseases.

Acta neuropathologica·2000

See all related articles