Testis-brain RNA-binding protein (Translin) is primarily expressed in neurons of the mouse brain

The subcellular location(s) of the DNA- and RNA-binding protein, Testis-Brain RNA-Binding Protein (TB-RBP)/Translin in mouse brain has been determined in paraffin sections by immunocytochemistry with an affinity purified antibody to mouse recombinant TB-RBP. Nuclear staining was frequently seen in neurons throughout the brain, but no TB-RBP/Translin was detected in many of the neurons in superficial layers of the cerebral cortex and in some cells of the cerebellum. Cytoplasmic staining extending into the dendrites was seen in large neurons such as pyramidal neurons in Layer 5 of the cortex and magnocellular neurons of the hypothalamus or the brainstem raphe.