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Updated: Oct 24, 2026

Short-Duration Hypothermia Induction in Rats using Models for Studies examining Clinical Relevance and Mechanisms
Published on: March 3, 2021
Dehydration modifies somal CRH immunoreactivity in the rat hypothalamus: an immunocytochemical study in the absence
1Neuroscience Graduate Program and the Department of Biological Sciences, Hedco Neuroscience Building, MC 2520, University of Southern California, Los Angeles, CA 90089-2520, USA.
Abstract:
Corticotropin-releasing hormone immunoreactive (CRH-ir) neurons were examined in the hypothalamus of euhydrated and dehydrated rats without using colchicine. CRH-ir cells were observed in the lateral hypothalamus, retrochiasmatic, and in magnocellular parts of the supraoptic and paraventricular hypothalamic nucleus (PVH) in dehydrated but not euhydrated animals. However, CRH-ir neurons were decreased in the medial parvicellular part of the PVH. These results indicate that altered CRH mRNA levels previously reported in dehydrated animals translate into changes in peptide immunoreactivity.
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