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[LPS receptor expression and lps binding capacity of monocytes and macrophages in swine]
1Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Fachbereich Jena.
Abstract:
The expression of the lipopolysaccharide (LPS) receptor CD14, being of importance for an effective immune response, and the direct measurable binding of LPS molecules by alveolar macrophages (AM), peritoneal macrophages (PM) and peripheral blood mononuclear cells (PBMC) of swine were determined. Additionally, the influence of an experimental intrabronchial infection with Pasteurella multocida on these parameters was investigated. Whereas the AM and the PM differed only negligibly regarding their share of CD14 bearing and LPS binding cells, in contrast to AM the intensity of the LPS-binding of PM was significantly higher. Among the PBMC, two populations could be detected which expressed the CD14 with different intensity, whereas the LPS-binding of both populations was similarly pronounced. Following infection of the animals, AM showed an unchanged percentage of CD14 positive cells and simultaneously an increased CD14 receptor expression. On the other hand, the percentage of the LPS-binding cells rose, while no changes of the intensity of LPS-binding were observable. The studies revealed that intrabronchial infection results in local and systemic alterations of CD14 expression and LPS-binding capacity of different cell populations. However, a direct correlation between the appearance of CD14- and LPS-binding does not seem to inevitably exist, indicating probably CD14-independent LPS-binding.
Insights
Investigating swine immune cells, this study found that Pasteurella multocida infection alters CD14 receptor expression and lipopolysaccharide (LPS) binding. These changes indicate potential CD14-independent LPS binding mechanisms in immune responses.
Area of Science:
- Veterinary Immunology
- Cellular Immunology
- Infectious Disease Immunology
Background:
- CD14 is a crucial receptor for lipopolysaccharide (LPS) recognition, essential for effective immune responses.
- Alveolar macrophages (AM), peritoneal macrophages (PM), and peripheral blood mononuclear cells (PBMC) are key immune cells involved in LPS detection.
- Understanding swine immune cell responses to LPS and bacterial infections is vital for animal health.
Purpose of the Study:
- To determine CD14 expression and LPS binding capacity in swine AM, PM, and PBMC.
- To investigate the impact of experimental Pasteurella multocida intrabronchial infection on these immune parameters.
- To explore the relationship between CD14 expression and LPS binding in different swine cell populations.
Main Methods:
- Flow cytometry was used to quantify CD14 expression and LPS binding on swine AM, PM, and PBMC.
- Experimental intrabronchial infection with Pasteurella multocida was performed in swine.
- Comparative analysis of CD14 expression and LPS binding was conducted between infected and control groups.
Main Results:
- PM showed significantly higher LPS binding intensity compared to AM, despite similar CD14 expression.
- PBMC exhibited two populations with differential CD14 expression but similar LPS binding.
- Following infection, AM displayed increased CD14 receptor expression and a rise in LPS-binding cells, without changes in binding intensity.
Conclusions:
- Intrabronchial Pasteurella multocida infection induces local and systemic alterations in CD14 expression and LPS-binding capacity in swine immune cells.
- The findings suggest a potential for CD14-independent LPS binding mechanisms.
- Immune cell responses to LPS are complex and can be modulated by infection, with varying roles for CD14.