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Updated: Aug 13, 2026

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
Published on: November 2, 2018
Abnormal binding pattern and composition of the NF-kappaB complex components are involved in increased TNF-alpha
1Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, FL 33136, USA.
Abstract:
B lymphocytes from mammary tumor bearers are cytotoxic against tumor targets and produce TNF-alpha. A greater stability of RNA and a decreased rate of RNA degradation was observed in B cells of tumor bearers compared to those of normal mice. The TNF-alpha promoter contains regions that bind NF-kappaB, which regulate the rate of transcription. Supershift assays for the NF-kappaB region showed that there are p50-p65 heterodimers and p50 homodimers in the nuclear extracts of the two types of B cells, while those from tumor bearers lack the c-Rel component that is present in normal B cells. These results indicate that abnormalities in binding and composition of the NF-kappaB complexes may be involved in the increased TNF-alpha production by B cells of tumor bearers.
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