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Updated: Aug 19, 2026

One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
Published on: June 18, 2016
Signaling pathway and pepsinogen secretion in Helicobacter pylori-infected human gastric adenocarcinoma
1Medical Biotechnology Laboratory, School of Medical Technology, Chang Gung University, Kweishan, Taoyuan, Taiwan, R.O.C.
Abstract:
Although in vitro studies have suggested that Helicobacter pylori not only attaches to cultured cells but also induces signal transduction events in host cells, the underlying mechanism of H. pylori action has yet to be fully investigated. In the present study, a cytotoxin-positive H. pylori was used to infect and examined for its effect on the stimulation of second messengers in human gastric adenocarcinoma (AGS). Results showed that H. pylori increased cytosolic free calcium concentration [Ca2+]i in host cells in a dose-dependent manner. The increase of [Ca2+]i was due to release from the intracellular Ca2+ store as well as entry to the extracellular Ca2+. H. pylori infection on host cells was also found to induce the generations of inositol phosphates, adenosine 3', 5'-cyclic monophosphate, and guanosine 3',5'-cyclic monophosphate, and to stimulate the secretion of pepsinogen.
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