Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Responses to single-dose thrombopoietin decrease with higher platelet counts in mice.

E Kelemen1, D Lehoczky, K Jakab

  • 1National Institute of Haematology and Immunology, Semmelweis University Medical School, Budapest, Hungary.

Acta Haematologica
|March 23, 1999
PubMed
Summary

Human serum and recombinant human TPO (rHuTPO) can induce thrombocytosis in mice. However, responses vary, suggesting an unknown endogenous mechanism regulates platelet production beyond TPO levels.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early Versus Delayed Autologous Stem Cell Transplantation and Interferon Maintenance in Multiple Myeloma: Single-Center Experience of 18 Years.

Transplantation proceedings·2016
Same author

The potential role of HLA-DRB1*11 in the development and outcome of haematopoietic stem cell transplantation-associated thrombotic microangiopathy.

Bone marrow transplantation·2015
Same author

Prevalence of physiological and pathological electrocardiographic findings in Hungarian athletes.

Acta physiologica Hungarica·2015
Same author

Intravenous antiarrhythmic doses of lidocaine increase the survival rate of CA1 neurons and improve cognitive outcome after transient global cerebral ischemia in rats.

Neuroscience·2011
Same author

Diagnostic accuracy of nerve fibre layer, macular thickness and optic disc measurements made with the RTVue-100 optical coherence tomograph to detect glaucoma.

Eye (London, England)·2010
Same author

Health anxiety in a non-population-based, pre-publicised glaucoma screening exercise.

Eye (London, England)·2009

Area of Science:

  • Hematology
  • Immunology

Background:

  • Thrombopoietin (TPO) is a key regulator of platelet production.
  • Altered thrombocytopoiesis can lead to abnormal platelet counts.
  • Previous studies (pre-1970) hinted at complex regulation of platelet levels.

Purpose of the Study:

  • To investigate the thrombocytosis-inducing capacity of human serum from individuals with altered thrombocytopoiesis.
  • To explore the role of endogenous mechanisms in response to thrombopoietin (TPO).
  • To analyze the effect of single versus repeated doses of recombinant human TPO (rHuTPO) on platelet counts in mice.

Main Methods:

  • Administering human serum samples from individuals with altered thrombocytopoiesis to recipient mice.
  • Administering single doses of recombinant human TPO (rHuTPO) to random-bred CFLP mice.

Related Experiment Videos

  • Mixing thrombopoietically active human sera with platelet-free normal serum.

    • Main Results:

    • Variable thrombocytosis observed in mice receiving human serum, even with identical samples.
    • Single doses of rHuTPO in mice showed variable responses, inversely correlating with initial platelet levels.
    • Mixing active human serum with normal serum reduced thrombocytosis-inducing capacity.
    • Repeated pharmacological doses of TPO obscured physiological control mechanisms.

    Conclusions:

    • Mouse response to exogenous rHuTPO is influenced by an unknown endogenous homeostatic mechanism.
    • Platelet count regulation involves more than just TPO levels.
    • Physiological control mechanisms are masked by repeated high-dose TPO administration.