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A very-high-purity von Willebrand factor preparation containing high-molecular-weight multimers
1HemaSure A/S, Gentofte, Denmark.tbaringt@post3.tele.dk
Background And Objectives:
We investigated large-scale production of very-high-purity von Willebrand factor (vWf) containing high-molecular-weight vWf multimers, using chromatography alone.
Materials And Methods:
Factor VIII (FVIII)-containing vWf was produced by initial separation of vWf from plasma by gel filtration followed by two ion exchange steps. Two virus inactivation steps were incorporated in the process.
Results:
The process resulted in FVIII-containing vWf preparations with a mean specific activity of 82 U vWf: collagen-binding activity per milligram protein (excluding added albumin) and with almost intact vWf multimer distributions.
Conclusions:
Very-high-purity, double virus-inactivated vWf preparations containing high molecular weight multimers were obtained on a production scale.