Pan-reactive platelet antibodies in post-transfusion purpura
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1Department of Clinical Immunology, Glostrup Hospital, Denmark.
Background And Objectives:
The mechanism for the destruction of a patient's own platelets in post-transfusion purpura (PTP) is unknown. In order to test the hypothesis that the destruction of autologous platelets in PTP is related to the presence of platelet antibodies with pan-reactivity, we investigated sera from patients with PTP.
Materials And Methods:
Sera from 12 patients with PTP were investigated for platelet antibodies by platelet-ELISA and monoclonal antibody-specific immobilization of platelet antigen assay.
Results:
During the thrombocytopenic phase, antibodies of IgG and IgM classes with pan-specificity against platelet GPIIb-IIIa, GPIb-IX and GPIa-IIa were found together with HPA alloantibodies. After recovery, the pan-specific antibodies disappeared or the extent of reactivity diminished, whereas the IgG HPA alloantibodies persisted.
Conclusion:
These findings provide evidence that transient panreactive antibodies may be responsible for the autologous platelet destruction in PTP.
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