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Human cts18.1 gene: chromosomal localization and PH-domain analysis.

H S Kim1

  • 1Department of Psychiatry, Warneford Hospital, University of Oxford, UK.

Genes & Genetic Systems
|March 23, 1999
PubMed
Summary

The human cts18.1 gene, homologous to cytohesins, is located on chromosome 19. Evolutionary analysis reveals negative selection acting on the pleckstrin-homology domain of cytohesin family genes.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • The cytohesin gene family plays crucial roles in cellular processes.
  • The human cts18.1 gene shares significant homology with known cytohesin genes.
  • Understanding the evolutionary pressures on these genes is vital for comprehending their functions.

Purpose of the Study:

  • To determine the chromosomal localization of the human cts18.1 gene.
  • To investigate the evolutionary dynamics of the pleckstrin-homology (PH) domain within the cytohesin gene family.
  • To establish the phylogenetic relationship of cts18.1 with other cytohesin family members.

Main Methods:

  • Polymerase Chain Reaction (PCR) analysis using a human monochromosomal somatic cell hybrid DNA panel for gene localization.
  • Analysis of synonymous and nonsynonymous substitution diversity to infer selection pressures.
  • Phylogenetic tree construction using the neighbor-joining method based on cDNA sequences of the PH domain.

Main Results:

  • The human cts18.1 gene was successfully mapped to chromosome 19.
  • Evidence of negative selection was identified within the PH domain of the cytohesin gene family.
  • Phylogenetic analysis indicated a closer evolutionary relationship between cts18.1 and cytohesin-2 compared to CLM-2.

Conclusions:

  • The cts18.1 gene is a member of the cytohesin gene family located on human chromosome 19.
  • The PH domain of cytohesins has undergone evolutionary constraints, suggesting functional importance.
  • cts18.1 and cytohesin-2 represent a distinct evolutionary branch within the studied cytohesin family.

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