Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Cancer chemo-endocrine therapy and its cell biological basis].

I Nishiya1

  • 1Morioka Red Cross Hospital, Japan.

Human Cell
|March 23, 1999
PubMed
Summary

Chemo-endocrine therapy impacts cancer cell cycles, with cisplatinum (CDDP) affecting DNA in ovarian cancer cells. Resistant cells show unique cell cycle shifts and DNA repair responses to CDDP treatment.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell proliferation and hCG secretion in human choriocarcinoma treated withmtx and it's insulin enhances.

International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics·2018
Same author

Nitric oxide mediates inhibitory effect of interleukin-1beta on estrogen production in human granulosa-luteal cells.

The journal of obstetrics and gynaecology research·2001
Same author

Telomerase and proliferative activity in placenta from women with and without fetal growth restriction.

Obstetrics and gynecology·1999
Same author

Telomerase activity in human chorionic villi and placenta determined by TRAP and in situ TRAP assay.

Placenta·1998
Same author

[Early phase II trial of oral etoposide administered for 21 consecutive days in patients with cervical or ovarian cancer. ETP 21 Study Group--Cervical-Ovarian Cancer Group].

Gan to kagaku ryoho. Cancer & chemotherapy·1998
Same author

Unscheduled expression of cyclins by anti-cancer drug exposure.

Human cell·1998

Area of Science:

  • Cell biology and molecular oncology
  • Cancer pharmacodynamics
  • Flow cytometry applications

Context:

  • Ovarian cancer is characterized by uncontrolled cell proliferation.
  • Flow cytometry (FCM) enables quantitative analysis of cell cycle parameters.
  • cis-diamminedichloroplatinum(II) (CDDP) is a key treatment for ovarian cancer.

Purpose:

  • To investigate the cell biological and molecular effects of chemo-endocrine therapy.
  • To analyze the relationship between CDDP cytotoxicity, cell cycle perturbations, and DNA damage in ovarian cancer cells.
  • To compare CDDP responses in sensitive (KFt) and resistant (KFr) ovarian adenocarcinoma cell lines.

Summary:

  • CDDP treatment induced a decrease in G1 phase cells and an increase in S phase cells in both sensitive and resistant ovarian cancer cells.
  • Resistant KFr cells exhibited a transient S-phase block with a G-C base pair preference, followed by DNA repair, and some cells showed later G2/M phase accumulation.
  • Bromodeoxyuridine (BrdU) labeling supported differences in drug sensitivity and resistance, suggesting damaged cells may re-enter the cell cycle.

Impact:

  • Provides insights into mechanisms of CDDP resistance in ovarian cancer.
  • Highlights the utility of multi-parameter FCM for analyzing drug-induced DNA damage and cell cycle kinetics.
  • Suggests potential strategies for targeted chemotherapy, including combining cytotoxic drugs with estrogen to enhance apoptosis and interfere with DNA repair.

Related Experiment Videos