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Related Experiment Videos

[Anticancer susceptibility test method using general-purpose dissolved oxygen meter].

J Arai1, K Yamada, M Yasuda

  • 1Department of Anatomy II, Jikei University School of Medicine.

Human Cell
|March 23, 1999
PubMed
Summary
This summary is machine-generated.

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[A case of severe HELLP syndrome with eclampsia].

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Confocal laser microscopy of dystrophin localization in guinea pig skeletal muscle fibers.

The Journal of cell biology·1992
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Limited arthrodesis for the rheumatoid wrist.

The Journal of hand surgery·1992
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Rheumatoid wrists treated with synovectomy of the extensor tendons and the wrist joint combined with a Darrach procedure.

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Nucleotide sequence and presumed secondary structure of the internal transcribed spacers of rDNA of the pea aphid, Acyrthosiphon pisum.

Comparative biochemistry and physiology. B, Comparative biochemistry·1992

This study introduces a novel dissolved oxygen measuring system to assess anticancer drug susceptibility by monitoring cellular respiration. The new bare oxygen electrode system offers rapid and accurate results comparable to conventional methods.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Analytical Chemistry

Context:

  • Drug susceptibility testing is crucial for effective cancer treatment.
  • Conventional methods can be time-consuming.
  • Monitoring cellular metabolic changes offers a rapid alternative.

Purpose:

  • To develop and evaluate a novel dissolved oxygen measuring system for rapid drug susceptibility testing.
  • To utilize cellular respiration volume as a metabolic indicator.
  • To compare the system's performance against the MTT assay.

Summary:

  • A new bare oxygen electrode system was developed to measure dissolved oxygen concentration changes, reflecting cellular respiration volume.
  • The system was validated using HL-60 cells against various anticancer drugs, showing results comparable to the MTT method.

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  • A multi-type electrode plate for 96-well plates was created for easier clinical evaluation.

    • Impact:

    • Provides a rapid, accurate, and potentially more accessible method for anticancer drug susceptibility testing.
    • Facilitates quicker treatment decisions in clinical settings.
    • Advances the field of cell-based metabolic monitoring for drug efficacy assessment.