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Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
13:38

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

Monitoring simultaneous subcellular events in vitro by means of coherent multiprobe fluorescence

D R Plymale1, J R Haskins, F A de la Iglesia

  • 1Pathology and Experimental Toxicology Department, Parke-Davis Pharmaceutical Research, Ann Arbor, Michigan 48105, USA.

Nature Medicine
|March 23, 1999
PubMed
Abstract

No abstract available in PubMed .

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...