Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Effect of phosducin on opioid receptor function.

R Schulz1, A Wehmeyer, K Schulz

  • 1Institute of Pharmacology, Toxicology and Pharmacy, University of Munich, München, Germany. shulz@pharmtox.vetmed.unimuenchen.de

The Journal of Pharmacology and Experimental Therapeutics
|March 23, 1999
PubMed
Summary

Phosducin (Phd) enhances opioid efficacy by inhibiting G protein GTPase activity and stabilizing signaling complexes. It also reduces opioid receptor internalization, impacting cellular responses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Venous thromboembolism risk assessment and prophylaxis in hospitalised medical patients in the Cape Town metropole, South Africa.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2022
Same author

Medicine-use evaluation of doxazosin prescribing to inform formulary recommendations.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2019
Same author

Sentinel lymph node biopsy vs. pelvic lymphadenectomy in early stage cervical cancer: is it time to change the gold standard?

Gynecologic oncology·2009
Same author

Early experience of a fall and fracture prevention clinic at Mayo General Hospital.

Irish journal of medical science·2009
Same author

Nasopharyngeal amyloidosis: an unusual cause for epistaxis.

The Journal of laryngology and otology·2009
Same author

Introduction. Equitation Science.

Veterinary journal (London, England : 1997)·2009

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pharmacology

Background:

  • Phosducin (Phd) is a phosphoprotein that regulates G protein function through binding to Gbetagamma subunits.
  • G protein signaling pathways are crucial for opioid receptor function, including adenylyl cyclase (AC) activity and receptor internalization.

Purpose of the Study:

  • To investigate the effect of Phosducin (Phd) on opioid receptor signaling, specifically adenylyl cyclase (AC) activity and receptor internalization.
  • To elucidate the mechanisms by which Phd influences opioid efficacy in cellular models.

Main Methods:

  • Utilized NG 108-15 hybrid cells stably expressing Phosducin (Phd).
  • Assessed adenylyl cyclase (AC) activity and cAMP generation in response to opioid agonists.
  • Measured [35S]guanosine-5'-O-(gamma-thio)-triphosphate incorporation and GTPase activity to study G protein activation.

Related Experiment Videos

  • Investigated opioid receptor internalization using mu-opioid receptors fused to green fluorescence protein and confocal microscopy.

    • Main Results:

    • Phosducin (Phd) overexpression increased the efficacy of opioids in inhibiting cAMP generation, despite inhibiting opioid-stimulated GTPase activity.
    • Phd significantly reduced the incorporation of [35S]guanosine-5'-O-(gamma-thio)-triphosphate and inhibited opioid-stimulated GTPase activity.
    • Phosducin (Phd) attenuated opioid-induced internalization of mu-opioid receptors, as observed through microscopy.

    Conclusions:

    • Phosducin (Phd) enhances opioid efficacy by inhibiting GTPase activity, which likely stabilizes the Gi-GTP complex and enhances AC inhibition.
    • The scavenging of Gbetagamma subunits by Phosducin (Phd) contributes to the observed attenuation of opioid receptor internalization.
    • These findings suggest Phosducin (Phd) plays a significant role in modulating G protein-coupled receptor signaling and opioid responses.