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Transepithelial capacitance decrease reveals closure of lateral interspace in A6 epithelia.

W Van Driessche1, R De Vos, D Jans

  • 1Laboratory of Physiology, K.U. Leuven, Campus Gasthuisberg, B-3000 Leuven, Belgium.Willy.VanDriessche@med.KULeuven.ac.be

Pflugers Archiv : European Journal of Physiology
|March 23, 1999
PubMed
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Cell swelling transiently decreases transepithelial capacitance (CHFT) by closing the lateral interspace. Recovery of CHFT correlates with regulatory volume decrease, indicating a link between cell volume regulation and membrane capacitance measurements.

Area of Science:

  • Cell biology
  • Biophysics
  • Membrane physiology

Background:

  • Transepithelial capacitance (CT) measurements are crucial for understanding cell membrane properties.
  • High-frequency transepithelial capacitance (CHFT) reflects apical and basolateral membrane capacitance in series.
  • Cell swelling dynamics and its impact on membrane capacitance require further investigation.

Purpose of the Study:

  • To investigate the relationship between cell swelling and transepithelial capacitance (CT).
  • To elucidate the mechanisms underlying changes in high-frequency transepithelial capacitance (CHFT) during cell swelling.
  • To explore the correlation between CHFT recovery and regulatory volume decrease (RVD).

Main Methods:

  • Sine wave method to measure transepithelial capacitance (CT) at 4.1 kHz (CHFT).

Related Experiment Videos

  • Induction of cell swelling by altering basolateral osmolality.
  • Impedance spectroscopy to obtain frequency dependence of CT (4 Hz–22 kHz).
  • Electron microscopy to observe lateral interspace (LIS) changes.

    • Main Results:

    • Cell swelling transiently decreased CHFT, with maximum decrease at 30s and recovery within 3-4 min.
    • CHFT decrease (DeltaCHFT) was dependent on the magnitude of osmotic perturbation and culture time.
    • Electron microscopy revealed correlation between CHFT changes and lateral interspace closure.
    • CHFT recovery was linked to regulatory volume decrease (RVD), inhibited by Gd3+.

    Conclusions:

    • DeltaCHFT is primarily caused by the closure of the lateral interspace (LIS) during cell swelling.
    • LIS closure increases access resistance to the basolateral membrane, reducing its apparent area at high frequencies.
    • Capacitance measurements, particularly CHFT, provide insights into cell volume regulation and membrane dynamics.