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Short-term regulation of endothelial Na(+)-K(+)-pump activity by cGMP: a 133Cs magnetic resonance study
1National Research Council, Institute for Biodiagnostics, Winnipeg, Manitoba, Canada. gruwel@ibd.nrc.ca
Abstract:
The effect of nitric oxide radicals (NO) on the activity of porcine aortic endothelial Na(+)-K(+)-ATPase is reported. Measurements were made using an in vitro cell system and 133Cs magnetic resonance (NMR). It is shown that NO, through stimulation of guanylate cyclase, results in a reduction of pump activity. Similar observations were made using 8-Br-cGMP. Measurement of the cytosolic volume indicated no changes in volume during incubation with 8-Br-cGMP. Our measurements indicate a continuous regulation of endothelial Na(+)-K(+)-ATPase activity by endogenous NO. This regulation could be removed by L-NAME, resulting in a small increase in pump activity.